Precision species identification for rapid environmental assessment
Precision species identification for rapid environmental assessment
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If you have any questions about this product, it’s suitability for your project, or anything else, book a free consultation with one of our molecular ecologists.
TaxaReveal™ Fungi characterises the aquatic mycobiome from eDNA water samples, including aquatic hyphomycetes, pathogenic oomycetes, and broader fungal diversity. Applications include aquatic hyphomycete community assessment, fungal water quality indicators, and general mycobiome profiling. Note: where Batrachochytrium dendrobatidis, B. salamandrivorans, or Aphanomyces astaci detection is the primary objective, the relevant TaxaScreen qPCR assays are more appropriate.
Target locus: Internal Transcribed Spacer regions 1 and 2 (ITS1 and ITS2) of the nuclear ribosomal RNA operon, the formal fungal DNA barcode. We utilise long-read metabarcoding for this assay to cover both the ITS1 and ITS2 regions as it increases species-specific results.
Known limitation: ITS copy number varies across fungal taxa by two to three orders of magnitude, meaning relative read abundance is non-proportional to biomass without normalisation. Standard fungal ITS primers also show reduced amplification efficiency against Batrachochytrium so where Bd or Bsal detection is a survey objective then TaxaScreen qPCR assays should be used in parallel or in preference.
